r/unclebens • u/wavyy_dreamer • 18h ago

Question MSS to agar no growth

I’m a new grower and I inoculated some UB bags and some pre bought malt extract agar plates with a blue meanie cubensis mss 8 days ago. I inoculated using a diy laminar flow hood and I’m keeping everything stored at a solid 76-78 degrees Fahrenheit using a temperature monitor and a humidifier on low to make sure nothing dries out keeping it at around 55% RH. Nothing. All the plates look the exact same from when they came. I messed up my first plate underestimating the pressure needed to push out the spores and ended up spraying some everywhere, but the rest only got one or two drops straight from the syringe no needle, wrapped in micropose parafilm. I also left two plates empty. One that I immediately sealed after opening the bag they came in, and one I left in front of my flow hood for about two minutes. Absolutely nothing. Nothing from the grain either. I used 3 uncle Ben’s and 3 off brand, all whole grain brown with no other additives, all being stored together with monitored temperature and humidity. I know mss syringes can take a while bc the spores need to germinate but I thought after a week I would’ve seen SOMETHING, even contamination. Any advise? Is it possible I got a dead syringe? There’s very clearly a lot of spores in the syringe and I shook it up pretty good before inoculating and I’ve only ever heard good things about this vendor. Any advise? Is it possible I got a dead syringe? There’s very clearly a lot of spores in the syringe and I shook it up pretty good before inoculating and I’ve only ever heard good things about this vendor. Could it be the agar plates? I don’t have a pc yet which is why I opted to buy the pre poured plates. Honestly I’m just about to get a LC syringe and try again to compare results and colonization speeds

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u/ConfidenceLopsided32 17h ago

Spores can germinate in less than 8 days but they can also take up to 45 days, sometimes 2 months even. It is essentially random. They usually take their sweet ass time.

If you don't see contamination by now, then you're still good, they just need more time to germinate. Once they germinate you'll see them start growing out quickly.

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u/wavyy_dreamer 17h ago

Well I’m glad contamination is less likely of an issue but mannn, I know growing is like 90% patience but ts is hard😭I already went ahead and bout a few lc syringes💀

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